Abstract

ANTICANCER ACTIVITY AND ANT DIABETIC ACTIVITY OF ASPERGILLUS STEREUS AF1

K. Daniel and M. Guravaiah*

Abstract

Microorganisms produce secondary metabolites which may have shown antimicrobial activity acting against certain pathogenic microorganisms. Streptomyces are saprophytic gram positive bacteria which have the ability to produce vast number of structurally and functionally. The MTT Cell Proliferation Assay measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, the reduction in cell viability. MTT Assay is a colorimetric assay that measures the reduction of yellow 3-(4,5- dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase. The in vitro cytotoxicity assay on human cervical cancer cell line (HeLa) revealed that the secondary metabolite had the strongest cytotoxicity with IC50 of 4.9 μg/ml.

Keywords: secondary metabolites, Streptomyces, MTT Assay and Heal cells.