Abstract

ANTIBACTERIAL ACTIVITY OF VITEX DONIANA (VERBENACEAE) BARK EXTRACTS AGAINST TWO BACTERIAL STRAINS PRODUCING OF β-LACTAMASES AT EXTENDED SPECTRUM (ESBL)

Ouattara Abou*, Touré Abdoulaye, Yeo Sounta Oumar, Golly Koffi Julien, Coulibaly Adama and Ouattara Karamoko

Abstract

Vitex doniana has been reported to be used in treatment of various diseases. The aim of our study was to evaluate the antibacterial properties of various extracts from the bark of V. doniana on Escherichia coli and Klebsiella pneumoniae, two strains of betalactamase producing extended spectrum (ESBL). The method of wells in the agar was used to test the sensitivity of bacterial strains while the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by the dilution method in liquid medium associated with the spread on gelose. The methanolic, and ethyl acetate extracts gave inhibition zone diameters between 11 and 19 mm on the strains tested. Moreover, these extracts showed bactericidal powers on E. coli ESBL and K. pneumoniae ESBL. Methanolic extract recorded an MBC of 142.84 mg/mL on both germs. As for ethyl acetate extract, the MBCs obtained were 6.25 mg/mL (E.coli ESBL) and 12.5 mg/mL (K. pneumoniae ESBL). These results justify certain ethnopharmarcological uses of V. doniana.

Keywords: Vitex doniana, bactericidal, Escherichia coli ESBL, Klebsiella pneumoniae, ESBL.