Abstract

INTERACTION AND PROTEOMIC ANALYSIS OF HEPATITIS B VIRUS INFECTED CELL LINE

*Ali Mohammed Abdulmusen

Abstract

A major obstacle to the research on the development of drug and genebased therapies for HBV infections has been the lack of an efficient cell culture system or a readily available small-animal model, permissive for viral infection and replication. Lack of a robust in vitro cell culture system has seriously hampered the progress of HBV research. Development of high throughput proteomics approach provides a new tool to study the pathogenesis of HBV and identification by molecular markers. Proteomics has been used to characterize the molecular events occurring in various disease processes. In this study the two dimensional electrophoresis (2-DE) followed by mass spectrometry (MS) analysis approach was used for identification and comparative expression profiles of protein level that may be associated with HBV infection. The Mascot analysis identified the proteins as envelope glycoprotein and Non structural protein 5 which were highly unregulated after viral infection. Such approaches are expected to establish the molecular definition of the HBV infection and contribute to the discovery of diagnostic markers and therapeutic targets.

Keywords: HBV, Hela, 2D electrophoresis, MALDI-TOF-MS.