Abstract

PHARMACOGNOSTIC STUDIES, EVALUATION OF EX-VIVO THROMBOLYTIC AND INVITRO ANTIOXIDANT ACTIVITIES OF LEAVES OF GUAIACUM OFFICINALE

Vijetha Pendyala*, Vidyadhara Suryadevara, Vineela Sathuluri and Davidwilson Perli

Abstract

The present study was an attempt to investigate macroscopic and microscopic characters, chemical nature, thrombolytic and antioxidant activity of Guaiacum officinale. Macroscopic, microscopic, qualitative phytochemical analysis, physiochemical analysis, extractive values in ethanol and water of the leaves were done. Macroscopic and microscopic study showed distinct morphological characteristics in the leaf. Physiochemical analysis of leaf powder revealed total ash 5%, acid-insoluble ash 2.7% alcohol soluble extractive 22.6%, water soluble extractive 31.4%. Pharmacognostic study of leaf is helpful in sample identification and to ensure quality and purity standards of Guaiacum officinale. We focused on the thrombolytic activity and antioxidant activity of Guaiacum officinale leaf extract using a simple in-Vitro clot lysis and reducing power assay methods respectively. The Results exhibited a maximum of 81.84% clot lysis at 600 μg/ml concentration in 72 hrs of incubation. The findings indicated that concentrations of leaf extract enhanced the percentage of clot lysis in dose dependent manner along with the incubation time factor. However, streptokinase SK a reference standard and water were used as a positive and negative control showed clot lysis maximum 91.77% and 4.22% in 72 hrs of incubation respectively. The antioxidant activity of the chloroform and hydroalcoholic extracts was found to be significant when compared with absorbance of standard ascorbic acid.

Keywords: Guaiacum officinale, Pharmacognosy, Microscopy, Anti-oxidant, Thrombolytic.