Abstract

IN-VITRO STUDIES ON ANTI DIABETIC AND WOUND HEALING ACTIVITY OF CHLOROXYLON SWIETENIA LINN (RUTACEAE) LEAF EXTRACT

B. Nagaraju*, H. Ramana, N. Shiva Krishna, M. Bhaskar and P. Venkateshwarao

Abstract

The plant chloroxylon swietenia linn popularly known as Ceylon Satin wood as branched perennial herb (rutaceae) and distributed in peninsular India, Srilanka. It shows potential herbal remedy in the treatment of chronic nebritis, cancer, diabetes, convulsions and jaundice. Though the plant is used for multiple purposes still proper pharmacognostic standardization as not been established for the leaves part. In microscopic studies it had revealed the presence of abundant xylem fibers and xylem vessels. The successive solvent extraction of leaves showed the phytoconstituents like flavanoids, saponins, glycosides, tannins and phenols. They were further confirmed by TLC analysis. The TLC studies showed the presence of chemical constituents with colored spots and Rf values. In Ova wound healing activity on fertilized egg was performed by making a window of 1cm2 on fertilized eggs and sterile cellulose discs loaded with aqueous. The plant extract had shown promising the results for anti-diabetic activity with glucose absorption study method at 1000 mg/ml, glucose oxidized method 100 mg/ml and wound healing activity by chorioallantoic membrane method at 600 mg/ml.

Keywords: Diabetes, Wound healing, Flavonoids, Glucose absorption study, TLC.