Abstract

IN VITRO REGENERATION OF COMMELINA DIFFUSA BURM. F. USING NODAL EXPLANTS

A. V. P. Karthikeyan, I. Sudan* and R. Satheeshkumar

Abstract

In Vitro Regeneration of plantlets has been achieved in an importantmedicinal plant of Commelina Diffusa (Commelinaceae) through nodalexplants. The nodal explants were cultured on MS mediumsupplemented with different concentrations of BAP (5 μM -25 μM)and KIN (5 μM -25 μM) alone. Among the two cytokinins tested, 10μM of BAP was found to induce more number of shoots (2.75 ± 0.47)than 10 μM KIN (3.2 ± 0.41). A maximum number of shoots wasinduced from all the various concentrations of BAP and KIN. Thehighest frequency of 90% shoot induction was observed on MS basalmedium supplemented with 10μM BAP and 10 μM KIN alone. Forroot induction regenerated shoots were transferred to the half strength MS basal mediumsupplemented with different concentration of IBA and IAA. The maximum numbers of roots(15.6 ± 1.48) were obtained from 6 μM IAA with mean root length of 6.7cm. The plantletswere successfully transplanted to the paper cup for hardening and then it was transferred tothe field with 85% survival rate.

Keywords: Commelina Diffusa Burm. f., nodal explants, BAP, KIN, IAA, IBA, MS medium.