Abstract

BIOCHEMICAL STUDIES OF FRESH WATER FEMALE CRAB BARYTELPHUSA GUERINI INOCULATED WITH E.COLI AND STAPHYLOCOCCUS AUREUS

CH. Tulasi Mastanamma*

Abstract

Barytelphusa Guerini revealed the presence of carbohydrates, Lipids, the enzymes prophenoloxidase, lysozyme in the haemolymph. Not all haemocytes were positive to the various cytochemical tests, implying that the haemocytes of Barytelphusa Guerini vary in their physiological role. Carbohydrate positive granules were observed in less number of haemocytes of Barytelphusa Guerini. The nature of the haemocytes positive to PAS staining and the nature of the carbohydrate could not be ascertained. The quantitative estimation of total carbohydrates was made in the crab, Barutelphusa gueriniat 2hrs, 6hrs, 12hrs, 24hrs and 48 hrs in control, control injured female crabs challenged with Gram negative (E.coli) and Gram positive (S.aureus) bacteria. Basing on the results it was observed that the carbohydrate content decreased gradually from 2 hrs to 48 hrs and the percentage of decrease is lowest i.e 21.6% in female crab challenged with S.aureus. it was observed that the protein content increase was moderate in the hemolymph of challenged crabs during 2hrs and 6hrs but reached maximum during 12hrs and started decreasing from 12hrs to 24 hrs and 48 hrs gradually in case of female crabs challenged with Gram positive and Gram Negative bacteria. it was observed that the lipid content increased gradually from 2 hrs to 24 hrs and decrease from 24 hrs to 48 hrs in female crabs challenged with E.coli and S.aureus.

Keywords: Barytelphusa Guerini, Biochemical studies, E.coli, Staphylococcus aureus