Abstract

STUDY OF ANTIMICROBIAL ACTIVITY OF MEDICINAL PLANT RHIZOSPHERE SOIL STREPTOMYCES ON SOME HUMAN SKIN PATHOGENS

*Raut R. A. and Kulkarni S. W.

Abstract

Actinomycetes are Gram-positive rod shaped, filamentous organisms which have ability to grow slowly as branching filaments. Most commonly soil isolated actinomycetes are of genus Streptomycetes. The aim of present study is to search antimicrobial activity produced by Streptomyces against some human skin pathogens. Total five rhizosphere soil samples of medicinal plants viz; Aloe barbadense (Aloevera), Emblica officinalis (Avala), Zingiber officinale (Ginger), Eucalyptus camaldulensis (Nilgiri) and Santalum album (Sandal) were collected from local area at Barshi, Dist. Solapur. M.S. India. Total thirty-two isolates were obtained and studied as actinomycetes on the basis of morphologically, culturally and biochemically characters. Out of these, nineteen isolates were identified from genus Streptomyces by MICRO-IS Software and also few by16srRNA analysis. The obtained isolates were tested for their antimicrobial activity by agar overlay technique against Staphylococcus aureus, Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa. The zone of inhibition around the growth of isolates were measured and recorded as a sensitive, while no zone of inhibiton around the isolate were consider as resistant to that test organism. Out of these isolates, five Streptomyces isolates were showed maximum antimicrobial activity against all tested skin pathogens Streptomyces enissocaesilis was showed antimicrobial activity against all pathogens except Pseudomonas aeruginosa. Streptomyces flavoviridis was showed antimicrobial activity against all tested human skin pathogens except Pseudomonas aeruginosa and Escherichia coli.

Keywords: Streptomyces, skin pathogens, agar overlay technique.