Abstract

ASSESMENT OF THE ANTI INFLAMMATORY EFFECT OF CINEOL ON CEREBRAL ISCHEMIA REPERFUSION I/R INJURY

Suhad Traiji Zamil*

Abstract

Ischemia and reperfusion in the brain brings a inflammating reaction that is possibly exacerbating primary stages of tissue wound. This study has been undertaken to inspect the potential neuroprotective action of cineol in the improvement of inclusive analytical I/R injury in rat model via interfering with inflammation. Twenty four adult Swiss albino rats had been chosen randomly in groups. For 1st group (sham group), rats endured the similar surgical and anesthesia processes as the controller group with no bilateral common carotid artery occlusion (BCCAO). While for 2nd control group (induced-untreated), rats endured half hour of global cerebral ischemia via BCCAO and then one hour of reperfusion. For 3rd group (Control – Vehicle), as controller collection but rats are taken day-to-day the vehicle of 1.8-cineol (10 mL/kg of 2% Tween 80 solution), p.o., then anesthesia and surgery with BCCAO for half hour followed by reperfusion for 1 hr were done. For 4th Group (1,8-cineol treated group), the rats received 1,8-cineol. The dose of 1,8- cineole was 100 mg/kg per oral (po) 1 h before induction ischemia. Then, anesthesia and surgery with BCCAO for 30 min, followed by reperfusion for 1 hr were done. Compared with the sham group, levels of IL-6 and TNF-α increased significantly (p< 0.05). The study results disclose that pretreating process with cineol possibly will enhance the overall cerebral ischemia-reperfusion injury by anti-inflammatory influence. We concluded that inflammatory cytokines are involved in global cerebral ischemia due to BCCAO effect. Cerebral ischemia reperfusion injury can be modified by cineol via its anti inflammatory effect.

Keywords: IL6, TNF-?, inflammation, cerebral ischemia/reperfusion injury, Cineol.