Abstract

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN- VITRO ANTIBACTERIAL ACTIVITY OF SOME MEDICINAL PLANTS AGAINST ESCHERICHIA COLI

Mahadev D. Jadhav, Kedar P. Deobhankar and L.R.Rathod

Abstract

The present study was undertaken to evaluate Preliminary Phytochemical and in vitro antibacterial activity of herbal extracts of selected medicinal plants against one pathogenic bacterium Escherichia coli. Methanol extracts of leaves of 11 medicinal plants viz. Allium sativum L., Ocimum sanctum L; Datura stramonium L; Eucalyptus globulesLabill. Calotropis procera (Ait.) R.Br, Azadirachta indica; A.Juss, Tridazpro cumbens L; Catharanthus roseus (L.) G.Don; Vitex negundoo L; Ricinus communis L.; Euphorbia hirta L; and fruit of Embilica officinalis Gaertn were used for study. First part of this study is dedicated toward isolation of bacteria and detection of bioactive compounds in selected plants. Preliminary phytochemical screening of methanol extracts of leaves of 11 medicinal plants. Flavonoid was the most ubiquitous of the chemical constituents in all plant extract except in case of leaf extracts of Datura Stramonium, Vitex negundo and Ricinus communis wherein it was conspicuously absent. Terpenoid was found to be absent in most of plant extracts except Allium sativum, Datura stramonium, Calotropis procera. . The leaf extract of Azadirachta indica was found to be significant zone of inhibition (24.6 mm) as compare to other selected medicinal plants.

Keywords: Escherichia coli, Medicinal Plants and Soxhlet extractor