Abstract

SCREENING AND PRODUCTION OF EXTRACELLULAR α – AMYLASE, PROTEASE AND GLUCOSE ISOMERASE FROM MESOPHILIC BACILLUS LICHENIFORMIS GINM-3 ISOLATED FROM AGRICULTURAL SOIL

Sanjay Parihar and Harshada Joshi*

Abstract

Microbial enzymes have proven their importance in bio-industries such as food, animal feed, leather, textiles as well as in bioconversions. The present study was conducted for extracellular screening and production of various microbial enzymes such as α-amylase, protease and glucose isomerase. An attempt was made to isolate mesophilic bacterial strains producing α-amylase, protease and glucose isomerase enzymes. Isolate GINM3 showed high yield production of these enzymes and the strain was later identified as Bacillus licheniformis by 16S rRNA partial genome sequencing. The production of enzymes was assayed in submerged fermentation condition. The maximum enzyme activity was found after 48 hours at pH 7. Enzymes activities for α- amylase was (0.316U/ml), protease (0.762U/ml), and glucose isomerase (0.369 U/ml).

Keywords: Protease, ?-amylase, glucose isomerase, mesophilic, enzyme activity.