Abstract

MICROBIAL DECOLORIZATION AND BIOREMEDIATION OF MELANOIDIN CONTAINING MOLASSES SPENT WASH

Dr. Madhavi. D. Rane*, Pritam Rathi and Ravi Rudra Tambe

Abstract

Effluents originating from distilleries which contain large amount of dark brown coloured wastewater called molasses spent wash (MSW). Dark brown colour of MSW is due to the presence of melanoidin pigment. It generally produced by „Millard‟ reaction and it is recalcitrant polymer. If dispose to untreated posses a great threat to environment. Melanodin is degrade or decolourise by using different microbial species. Microbial decolourization and degradation (COD) chemical oxygen demand was find to be dependent on the specific carbon and nitrogen source. The collection of indigenous bacterial cultures was performed to estimate its ability to degrade harmful pollutants present in molasses spent wash (MSW) and to understand its degradation potential through natural attenuation for which bacterial (Bacillus subtilis) and fungal strain (Fusarium solani) was utilized. From the following results 1.2% glucose/peptone concentrations were considered to be the optimized source for carbon and nitrogen for the organisms. Further, the degradation potential of the consortia in different concentrations of MSW along with its application on potted wheat plant was studied.

Keywords: Spent wash, indigenous, degradation, Fungus (Fusarium solani), Bacteria (Bacillus subtilis).