Abstract

UV SPECTROPHOTOMETRIC ESTIMATION OF EMBELIN AND VALIDATION OF DEVELOPED METHOD

* Sudani R.J¹, G. Vidyasagar²

Abstract

Embelia ribes burm f., also known as Vidanga, is one of the oldest herbs in Indian traditional medicine. Embelia ribes have a long history of use in ayurvedic system of medicine in various forms like churna, asava, aristha, lauha and taila. It is used mainly as an anthelmintic, carminative and stimulants. A simple and reproducible UV Spectrophotometric method has been developed for the estimation of Embelin in Vidanga churna formulations. Methanol was used as a solvent. Scanning wascarried out in the UV range of 200- 400 nm. Embelin showed maximum absorbance at 291 nm.Developed method showed good regression (r2= 0.9982± 0.0003)and the recovery of Embelin was in the range of 98.64 – 102.38%. The limit of detection and limit of quantitation were found to be 0.05μg/ml and 0.1571μg/ml respectively. The method was validated for precision, recovery, limit of detection and limit of quantitation. The proposed method being precise and sensitive can be used for detection, monitoring and quantification of Embelin in E. ribes.

Keywords: Vidanga, Embelin, UV Spectrophotometric