Abstract

ESTIMATION OF PROTEIN, CARBOHYDRATE AND LIPIDS IN FEMALE MUD CRAB SCYLLA SERRATA: CYTOCHEMICAL STUDIES AFTER INOCULATED WITH KLEBSIELLA PNEUMONIA AND BACILLUS CEREUS

*Saritha Subhashini

Abstract

Scylla serrata is having always a stress and threat from microbial organism that tries to cause infection. In the present study it was observed that the protein content increase was moderate in the hemolymph of challenged crabs during 2hrs and 4hrs but reached maximum during 12hrs and started decreasing from 12hrs to 24 hrs gradually in case of female crabs challenged with Gram positive and Gram Negative bacteria. The quantitative estimation of total carbohydrates was made in the crab, Scylla serrata at 2hrs, 4hrs, 8hrs, 12hrs, 16hrs, 20hrs and 24hrs in control, control injured and female crabs challenged with Klebsiella pneumonia and Bacillus cereus. Basing on the results it was observed that the carbohydrate content decreased gradually from 2 hrs to 24hrs and the percentage of decrease is highest 21.6% in female crab challenged with Bacillus cereus. The lipid content increased gradually from 2 hrs to 12 hrs and decrease from 12 hrs to 24hrs in female crabs challenged with Klebsiella pneumonia and Bacillus cereus. The percent of increase from 2hrs to 24 hrs is 19% in female crab challenged with Klebsiella pneumonia and 10% in female crab challenged with Bacillus cereus.

Keywords: Cytochemical studies, Scylla serrata, Klebsiella pneumonia and Bacillus cereus.