Abstract

DETERMINATION OF PHYTO-CONSTITUENTS IN TEPHROSIA PURPURA FLOWER EXTRTACT USING GC-MS

R. Kavitha* and A. M. Uduman Mohideen

Abstract

GC-MS has become a highly recommended tool for monitoring and tracking organic compounds in the environment. GC-MS is exclusively used for the analysis of esters, fatty acids, alcohols, aldehydes, terpenes etc. The aim of this study was to carry out for identification of phytocompounds from the methanolic extract of Tephrosia purpura flower by Gas chromatography and Mass spectroscopy (GC-MS). GCMS analysis of methanolic extract was done by standard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis revealed the presence of various compounds like tetradecane, Tetradecane, Pentadecane, Tetradecanoic Acid, Hexanoic Acid and tridecanoic acid in the methanolic extract of Tephrosia purpura. Hence, the Tephrosia purpura may use anticancer, anti-microbial activity, antioxidant and antiinflammatory activity due to the presence of secondary metabolites in the methanolic extract. These findings support the traditional use of Tephrosia purpura in various disorders.

Keywords: Gas chromatography and Mass spectroscopy, Tephrosia purpura, Phytochemistry.