Abstract

ANTI-MICROBIAL EFFECTS OF ANDROGRAPHOLIDE USING GIBBERLIC ACID, COLCHICINE, ABSCISIC ACID INDIVIDUALLY AND IN CONSORTIA AND ITS EXPRESSION ON THE ENZYME AMYLASE

Subhashini A.*, M. Jeevitha M. and H. Deepa. H.

Abstract

Andrographis paniculata, traditionally known as Kalmegh or “Neelavembu and commonly as “king of Bitters” belonging to the family. Three different concentrations of Stimulants (Gibberellic acid, Colchicine, Abscisic acid and consortia) were used for treatment after 40 days of transplantation. Sampling for expression analysis was done at 2hrs, 6hrs and 24hrs after foliar application. Cytological effect of treated A. paniculata plants and Cytotoxicity effect using onion bulbs was also studied. The result shows the highest levels of protein in Gb3 treated plants as recorded in 10mM concentration of 6hr treatment (185.13 and 175.3). Highest levels of carbohydrates was noticed in 10mM of 2hrs, 6hrs and 24hrs harvest periods as 117.98, 221.25 and 109.31. The highest levels of fatty acid in Gibberlic acid treated plants was recorded at harvest treatment 24hr in 10mM (0.130). It was lower when compared to untreated plants (0.178). In 15mM concentration, stimulation of bacteria and fungus was noticed at 6hr and 24hr. Similarly in 0.05% concentration, stimulation of fungi was recorded at 24hr. HPLC analysis of standard andrographolide showed three peaks with Retention time(Rt) of 2.5min. The A. paniculata extract of 2hr concentration of consortia showed two peaks at Retention time of 3min. The Gibberlic acid treated plant extract showed two peaks at Retention time of 3min in 24hr harvest periods of 15mM concentration. The Abscisic acid treated plant showed two peaks at Retention time of 3min in 24hr harvest period of 5mM concentration. In 24hr harvest period of 10mM concentration of ABA was also showed two peaks at Retention time of 3min and 5min. Whereas a single peak were observed at Retention time of 7min.

Keywords: Andrographis paniculata, Colchicine Gibberlic acid, Abscisic acid.