Abstract

ANTIBIOTIC SUSCEPTIBILITY PATTERN OF EXTENDED SPECTRUM Î’-LACTAMASE PRODUCING ESCHERICHIA COLI.

Parveen W.* and Subhashini A.

Abstract

Extended Spectrum β-Lactamase producing Escherichia coli have been demonstrated to increase resistance to antimicrobial agents. ESBL enzymes are capable of hydrolyzing oxyimino β-lactam compounds and contribute to high resistance to β-lactam antibiotics. The aim of this present study is to isolate and to identify the ESBL producing E. coli strains from UTI and Wound Infection. 67.3%, 17.3% of Urine and Pus samples were positive for E.coli strains. Out of 72 E.coli isolates 66.6%, 68%, 62.2% were resistant for Cefotaxime, Ceftriaxone, Ceftazidime respectively. The positive isolates from Primary screening test were further confirmed with phenotypic tests by performing Double Disc Diffusion and E-Test. 63.8% of clinical isolates were positive for Double Disc Diffusion and E-test. Current investigation reveals that E. coli isolates are multi-drug resistance and produce ESBL in large proportions.

Keywords: Extended Spectrum ?-Lactamse, Escherichia coli, Double Disc Diffusion Test, ESBL E-Test, Urinary tract Infection.