Abstract

AN EXTRACELLULAR ENZYME – PECTINASE: EFFICIENT PRODUCTION FROM THE BACTERIA BACILLUS SP.P15 ISOLATED FROM BANANA ORCHARD SOIL

Poovazhagi R. and Vijaya Ramesh K.*

Abstract

Pectinolytic enzyme can be applied in various industrial applications. Among the 27 bacterial strains isolated, 8 revealed pectinase activity from which we chose Bacillus sp. P15 based on significant zone of clearance. An attempt was made to optimize the partial purification methods by comparing lyophilisation (Concentrate) and ammonium sulfate precipitation followed by staining with Blue silver dye (Coomassie G-250). The lyophilized pectinase sample showed efficient enzyme activity and protein content. We propose to purify and commercialize this bacterial (Bacillus sp.P15) extracellular pectinase enzyme.

Keywords: Extracellular pectinase enzyme, Bacillus sp. P15, Lyophilization, Ammonium sulfate precipitation, Blue Silver Staining.