Abstract

BIOSYNTHESIS OF CHARACTERIZATION OF SILVER NANOPARTICLES USING SEEDS OF PONGAMIA PINNATA (L) PIERRE

Premalatha M., Hemamalini V.*, Anandhalakshmi J. and Bakyalakshmi G.

Abstract

The goal of research work is to synthesis and characterization of silver nanoparticles using seeds of Pongamia pinnata. Nanoparticles using green method show a clean, simple, less toxic and environmentally benign process. In this communication, silver nanoparticles were synthesized and characterized using the seed extract of Pongamia pinnata. The resultant biosynthesized silver nanoparticles characterized by UV-vis spectrophotometry shows the surface plasmon resonance band at 427 nm. FTIR-Fourier Transform Infrared spectrometer analysis was carried out to determine the capping agents in the seed extract. X-ray diffraction shows crystalline structure. TEM image divulges that silver nano particles are quite polydispersity, the size ranging from 10 nm to 20 nm with an average of 15 nm. The elemental analysis shows strong singal at 3keV that corresponds to silver ions and confirms the presence of metallic silver. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against gram positive and gram negative bacteria. Maximum and minimum zones of inhibition were noted against Staphylococcus aureus (20 nm), Escherichia coli (18 nm), respectively. This study reveals that silver nanoparticles possess good antibacterial activity 100μg/ml concentration.

Keywords: Bio synthesis, Pongamia pinnata, silver nanoparticles, TEM, antibacterial.