Abstract

IN VITRO ANTIBACTERIAL POTENTIAL OF SYNTHESISED SILVER NANOPARTICLES FROM LEAVES OF AVERRHOA BILIMB

Anandhalakshmi J., Bakyalakshmi G. and Hemamalini V.*

Abstract

Nanotechnology is a rapidly growing field and plays an important role in most of the advanced science, medicine, and technology areas. Plants are the major sources of different types of phytochemicals with numerous biomedical applications. This study focused on the phytosynthesis of silver nanoparticles (AgNPs) using the leaf extract of Averrhoa bilimbi L and their antibacterial potential. Phytochemical analysis revealed that the rich amount of biochemical present in leaf extract and these bio-surfactant molecules in the extract play a significant role both as stabilizing and reducing agents for converting silver nitrate into AgNPs. The silver nanoparticles synthesized were confirmed by their change of colour to dark brown due to the phenomenon of surface Plasmon resonance (SPR) and characterization was studied by UV-visible spectroscopy, Fourier-Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD) analysis. The morphology and size of synthesized silver nanoparticles were confirmed by Transmission Electron Microscopy (TEM) analysis with size range from 10 to 20 nm. The biosynthesized AgNPs were further studied for its anti-bacterial activity by using agar well diffusion and Minimum Inhibitory Concentration (MIC) methods at different concentrations against human pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa). AgNPs were showed potent antibacterial against all the pathogenic enteric bacteria tested. The AgNPs showed maximum efficacy against P.aeruginosa and E.coli. The AgNPs were found to be more susceptible to all the organisms than the aqueou

Keywords: Bio-Surfactant Molecules, AgNPs, Averrhoa bilimbi, SPR.