Abstract

GROWTH INHIBITORY AND CYTOTOXIC EFFECTS OF MIMOSINE ON HUMAN NEURONAL CELL LINES – IMR-32, U373-MG, SK-N-SH BY SRB ASSAY

Kannan R.* and Sibi P. Ittiyavirah

Abstract

Mimosine, a non-protein amino acid, isolated from Mimosa pudica was evaluated for its neuroprotective effects using human cell lines IMR-32, U373-MG and SK-N-SH. The cytotoxic potential was assessed by Sulforhodamine B assay and the parameters like GI50, TGI and LC50 were measured. The GI50 was found to be 55.2 and 37.3μg/ml for IMR-32 and U373-MG while that for standard Adriamycin it was less than 10μg/ml and no significant inhibition was noted on SK-N-SH cell lines. It was concluded that Mimosine exhibited a significant cytotoxic activity with fatality as evidenced by the LC50 values.

Keywords: Mimosine, SRB assay, IMR-32, U373-MG, SK-N-SH.