Abstract

EFFECT OF DIFFERENT CONCENTRATIONS OF METAL IONS ON Α-AMYLASE PRODUCTION BY BACILLUS LICHENIFORMIS DS3

D. Silpa, P. Brahmaji Rao and G. Kranthi Kumar*

Abstract

Ten bacterial strains were isolated from banana field soils collected from in the vicinity of Guntur, Andhra Pradesh, India. Isolation of bacteria was done by serial dilution method. Out of 10 isolates, DS3 showed the maximum clearance zone on Starch Agar medium. The identification of the isolate DS3 was confirmed by Bacillus licheniformis DS3 after 16S rRNA sequencing analysis. Further, different metal ions were supplemented into Starch agar media for amylase activity. Various metal ions CaCl2, CuSO4, MgSO4, MnSO4 and FeSO4 were supplemented into the medium at different concentrations of 0, 0.1, 0.2, 0.3, 0.4 and 0.5 %. Maximum amylase activity was recorded in the presence of Ca2+ (154.8 U/ml) at 0.4%. Among the metal ions tested FeSO4 showed the minimum enzyme activity (82.2 U/ml) at 0.2% concentration. Supplementation of metals on certain ions provides best growth of B. licheniformis DS3 and also increased the alpha amylase activity. It is thus Ca2+ may be considered as best ion for optimum growth and amylase production of B. licheniformis DS3. This study reports the addition of metal ions increased the production of amylase.

Keywords: Amylase, Bacillus licheniformis, Dinitrosalsylic acid (DNS), Metal ions.