Abstract

EVALUATION OF ANTIMICROBIAL EFFECT OF ALLIUM SATIVUM EXTRACT AND GOMUTRA

Sonia S., Vidhya A., Venkat Kumar S. and Dr. Jasmine John*

Abstract

This study was designed to test the antimicrobial activity of various solvent extracts of A. sativum and gomutra. The antimicrobial activity for the extracts was observed against the common wound infection causing bacterial strains Staphylococcus aureus and Pseudomonas sp and fungal strains Aspergillus niger and Penicillium sp. The antimicrobial activity was tested by agar well diffusion method. The A. sativum extract was prepared with and without skin using various solvents like ethanol, methanol and distilled water. Based on the different solvents used for extraction the extracts were labeled as GJA, GJM, GJE, GJWSA, GJWSM and GJWSE. Almost all the solvent extracts exhibited antibacterial and antifungal activity against the tested organism, when compared to other solvents aqueous extract is more effective. Also those extracts with skin showed increased zone of inhibition. Preliminary phytochemical analysis of the plants constituents were assessed by using qualitative methods. Test was conducted for the following active components: alkaloids, flavanoids, steroids, phlobatannin, cardiac glycosides, phenols, saponins, volatile oil and glycosides. FTIR analysis was done to find out different functional groups present in the extracts. HPLC and GCMS were also done. Also MIC value of the various solvent extracts of A. sativum was determined. The MIC value for GJA, GJWSA, GJE and GJWSE against Pseudomonas sp is 1, 10, 10 and 1 mg/ml respectively. The MIC for GJA, GJWSA, GJE and GJWSE against S. aureus is 100, 100, 100 and 1 mg/ml respectively.

Keywords: Garlic, phytochemical, antimicrobial activity, GCMS, FTIR, HPLC.