Abstract

PREPARATION OF SILK FIBROIN / NANOCHITOSAN BLENDS TOWARDS HIGH PERFORMANCE TISSUE ENGINEERING APPLICATIONS

S. Gokila and P. N. Sudha*

Abstract

Tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both Nanochitosan (NCS) and silk fibroin (SF) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the structure and related properties of NCS/SF Composites were examined. The prepared sample was then characterized using advanced analytical techniques such as FT-IR and XRD. The scaffold material was most suitable for osteoblast growth were determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, NCS /SF composites with different component were prepared and their structure was characterized. Changes in the internal structure of the NCS and SF mixture were observed, confirming that the mutual modification between the two components was complete and stable. This favors the early adhesion, growth and proliferation of MC3T3-E1 cells. Some of the assays studied in the cell line- MC3T3-E1 include MTT and Alizarin Red. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, NCS/SF is a good material fo tissue engineering.

Keywords: Silk fibroin, Nanochitosan, characteristics, cell culture, in vitro studies.